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mouse anti syx1  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse anti syx1
    Mouse Anti Syx1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+syntaxin/anti-Syntaxin/bio_rxiv__64898__2026__03__17__712223-279-26-29
    Average 93 stars, based on 58 article reviews
    mouse anti syx1 - by Bioz Stars, 2026-09
    93/100 stars

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    Control:

    Article Title: A targeted genetic modifier screen in Drosophila uncovers vulnerabilities in a genetically complex model of colon cancer
    Article Snippet: For western blot analysis, proteins were separated using 4–12% CriterionTM XT Bis-Tris Protein Gels (Bio-Rad #3450125). .. Primary antibodies used were Rabbit anti-phospho-AKT (1:1000, Cell Signaling Technology #4060), Mouse anti-diphospho-Erk (1:1000 Sigma-Aldrich #M8159) and Mouse anti-Syntaxin as loading control (1:1000 Developmental Studies Hybridoma Bank #8C3). .. The secondary antibodies were Anti-rabbit IgG, HRP-linked Antibody (1:1000, Cell Signaling Technology #7074) and Anti-mouse IgG, HRP-linked Antibody (1:5000, Cell Signaling Technology #7076).

    Article Title: A targeted genetic modifier screen in Drosophila uncovers vulnerabilities in a genetically complex model of colon cancer.
    Article Snippet: .. D ow nloaded from https://academ ic.oup.com /g3journal/article/13/5/jkad053/7070402 by guest on 27 January 2024 Technology #4060), Mouse anti-diphospho-Erk (1:1,000 Sigma-Aldrich #M8159), and Mouse anti-Syntaxin as a loading control (1:1,000 Developmental Studies Hybridoma Bank #8C3). .. The secondary antibodies were Anti-rabbit IgG, HRP-linked Antibody (1:1,000, Cell Signaling Technology #7074), and Anti-mouse IgG, HRP-linked Antibody (1:5,000, Cell Signaling Technology #7076).

    Article Title: A targeted genetic modifier screen in Drosophila uncovers vulnerabilities in a genetically complex model of colon cancer
    Article Snippet: For western blot analysis, proteins were separated using 4–12% Criterion XT Bis-Tris Protein Gels (Bio-Rad #3450125). .. Primary antibodies used were Rabbit anti-phospho-AKT (1:1,000, Cell Signaling Technology #4060), Mouse anti-diphospho-Erk (1:1,000 Sigma-Aldrich #M8159), and Mouse anti-Syntaxin as a loading control (1:1,000 Developmental Studies Hybridoma Bank #8C3). .. The secondary antibodies were Anti-rabbit IgG, HRP-linked Antibody (1:1,000, Cell Signaling Technology #7074), and Anti-mouse IgG, HRP-linked Antibody (1:5,000, Cell Signaling Technology #7076).

    Western Blot:

    Article Title: Pumilio differentially binds to mRNA 3' UTR isoforms to regulate localization of synaptic proteins.
    Article Snippet: .. For western blots, mouse anti-CSP (DSHB #DCSP-2 (6D6)), mouse anti-Synapsin (DSHB #3C11), mouse anti-Syntaxin (DSHB Cat#8C3), mouse anti-Discs large (DSHB Cat4D3), mouse antialphaTubulin (DSHB Cat#AA4.3), rabbit anti-ELAV (Carrasco et al, 2020), mouse anti-LaminC (DSHB Cat#LC28.26) and rabbit anti-Histone H3 (Abcam #Ab1791) were used at concentrations 1:150, 1:400, 1:250, 1:350, 1:100, 1:1000, 1:450, and 1:10,000, respectively. .. Peroxidase-conjugated antibodies were used at the following concentrations: protein A (Life Technologies #101023) 1:4000, anti-Flag (Sigma #A8592) 1:10,000.

    Article Title: Pumilio differentially binds to mRNA 3’ UTR isoforms to regulate localization of synaptic proteins
    Article Snippet: .. For western blots, mouse anti-CSP (DSHB #DCSP-2 (6D6)), mouse anti-Synapsin (DSHB #3C11), mouse anti-Syntaxin (DSHB Cat#8C3), mouse anti-Discs large (DSHB Cat4D3), mouse anti-alphaTubulin (DSHB Cat#AA4.3), rabbit anti-ELAV ( Carrasco et al ., 2020 ), mouse anti-LaminC (DSHB Cat#LC28.26) and rabbit anti-Histone H3 (Abcam Ab1791) were used at concentrations 1:150, 1:400, 1:250, 1:350, 1:100, 1:1000, 1:450 and 1:10,000, respectively. .. Peroxidase-conjugated antibodies were used at the following concentrations: protein A (Life Technologies 101023) 1:4000, anti-FLAG (Sigma #A8592) 1:10000.

    Article Title: Pumilio differentially binds to mRNA 3′ UTR isoforms to regulate localization of synaptic proteins
    Article Snippet: .. For western blots, mouse anti-CSP (DSHB #DCSP-2 (6D6)), mouse anti-Synapsin (DSHB #3C11), mouse anti-Syntaxin (DSHB Cat#8C3), mouse anti-Discs large (DSHB Cat4D3), mouse anti-alphaTubulin (DSHB Cat#AA4.3), rabbit anti-ELAV (Carrasco et al, ), mouse anti-LaminC (DSHB Cat#LC28.26) and rabbit anti-Histone H3 (Abcam #Ab1791) were used at concentrations 1:150, 1:400, 1:250, 1:350, 1:100, 1:1000, 1:450, and 1:10,000, respectively. .. Peroxidase-conjugated antibodies were used at the following concentrations: protein A (Life Technologies #101023) 1:4000, anti-Flag (Sigma #A8592) 1:10,000.

    other:


    Article Title: The NAD + precursor NMN activates dSarm to trigger axon degeneration in Drosophila
    Article Snippet: 1:5000 rabbit anti-GFP (Abcam, ab6556), 1:15,000 mouse anti-Tubulin (Sigma), 1:5000 rabbit anti-Tubulin (Abcam, T9026), 1:2000 rabbit anti-PncC (LubioScience, established in this study), 1:1000 mouse anti-FLAG (Sigma, F3165), 1:500 mouse anti-Syntaxin (DSHB, 8c3), 1:2000 anti-NAMPT (Merk, MABS465).



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    Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and <t>Stx18</t> protein bands from (C) quantified as described in (A), normalized to the respective <t>Syntaxin</t> values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
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    Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and <t>Stx18</t> protein bands from (C) quantified as described in (A), normalized to the respective <t>Syntaxin</t> values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
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    Image Search Results


    Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and Stx18 protein bands from (C) quantified as described in (A), normalized to the respective Syntaxin values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: Legionella employs the multimodal ubiquitination of Sec22b to modulate SNARE pairing

    doi: 10.1016/j.isci.2025.114341

    Figure Lengend Snippet: Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and Stx18 protein bands from (C) quantified as described in (A), normalized to the respective Syntaxin values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.

    Article Snippet: Anti-Stx18 mouse antibody , Santa Cruz Biotechnology , Cat# sc-293067; RRID:AB_10647235.

    Techniques: Ubiquitin Proteomics, Stable Transfection, Expressing, Infection, Immunoprecipitation, SDS Page, Western Blot, Software, Bacteria